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Real-Time PCR Assay Targeting IS <i>481</i> of <i>Bordetella pertussis</i> and Molecular Basis for Detecting <i>Bordetella holmesii</i>

1 May 2001

Abstract excerpt

Detection of Bordetella holmesii by a real-time PCR assay targeting IS481 of Bordetella pertussis is reported. Sequencing of IS481-specific PCR products from B. pertussis and B. holmesii isolates revealed sequence homology. Restriction fragment length polymorphism demonstrated a low copy number of IS481-like sequences in B. holmesii. These results, and culture of B. holmesii from patients with cough, suggest that...

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Identifiers

PMID: 11326023
DOI: 10.1128/jcm.39.5.1963-1966.2001

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