Comparison of Bordetella species identification among differing rt-PCR assays in the United States

Abstract excerpt

ABSTRACT In the United States, the general laboratory method for diagnosing pertussis, caused by Bordetella pertussis, is real-time PCR (rt-PCR) targeting insertion sequence 481 (IS 481 ). Other Bordetella species ( parapertussis, holmesii , and bronchiseptica ) can also cause a pertussis-like syndrome, and some commercial laboratory assays include the insertion sequence 1001 (pIS 1001 ) that can detect B....

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