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Harmonization of Bordetella pertussis Real-Time PCR Diagnostics in the United States in 2012

30 Oct 2014

Abstract excerpt

Real-time PCR (rt-PCR) is an important diagnostic tool for the identification of Bordetella pertussis, Bordetella holmesii, and Bordetella parapertussis. Most U.S. public health laboratories (USPHLs) target IS481, present in 218 to 238 copies in the B. pertussis genome and 32 to 65 copies in B. holmesii. The CDC developed a multitarget PCR assay to differentiate B. pertussis, B. holmesii, and B. parapertussis and...

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PMID: 25355770
DOI: 10.1128/jcm.02368-14

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