Article
Comparison of PCR, Culture, and Direct Fluorescent-Antibody Testing for Detection of <i>Bordetella pertussis</i>
1 Sept 1999
Abstract excerpt
We prospectively compared the performance of culture, direct fluorescent-antibody testing (DFA), and an in-house-developed PCR test targeting the repeated insertion sequence IS481 for the detection of Bordetella pertussis in nasopharyngeal swab specimens. We tested 319 consecutive paired specimens on which all three tests were performed. A total of 59 specimens were positive by one or more tests. Of these, 5 were...
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