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Novel Multitarget Real-Time PCR Assay for Rapid Detection of Bordetella Species in Clinical Specimens

22 Sept 2011

Abstract excerpt

A novel multitarget real-time PCR (RT-PCR) assay for the rapid identification of Bordetella pertussis, B. parapertussis, and B. holmesii was developed using multicopy insertion sequences (ISs) in combination with the pertussis toxin subunit S1 (ptxS1) singleplex assay. The RT-PCR targets for the multiplex assay include IS481, commonly found in B. pertussis and B. holmesii; IS1001 of B. parapertussis; and the...

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PMID: 21940464
DOI: 10.1128/jcm.00601-11

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