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Detection and Differentiation of Bordetella spp. by Real-Time PCR

7 Dec 2006

Abstract excerpt

Molecular detection of Bordetella pertussis DNA is a sensitive and specific method for the rapid diagnosis of pertussis. In this study, a new molecular assay for the detection and differentiation of Bordetella spp. based on automated DNA extraction and real-time PCR was evaluated. The analytical sensitivity of the new assay was determined by Probit analysis of serial dilutions of both cloned PCR products IS481...

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Share to Bacterial Infections and Vaccines
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PMID: 17151212
DOI: 10.1128/jcm.01303-06

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