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Real-Time LightCycler PCR for Detection and Discrimination of <i>Bordetella pertussis</i> and <i>Bordetella parapertussis</i>

1 May 2002

Abstract excerpt

Real-time PCR assays based on the LightCycler technology were developed for individual (simplex PCR) and simultaneous (duplex PCR) detection and discrimination of Bordetella pertussis and Bordetella parapertussis in clinical samples. The assays were evaluated with 113 specimens from patients with and without symptoms of pertussis. Results were compared to those from conventional culture and TaqMan real-time PCR....

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PMID: 11980949
DOI: 10.1128/jcm.40.5.1719-1722.2002

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