Article
Single nucleotide mismatch analysis using oligonucleotide probes synthesized on bacterial magnetic particle.
Biomolecular engineering - 1 Jul 2003
Ota Hiroyuki, Arakaki Atsushi, Tanaka Tsuyoshi, Takeyama Haruko, Matsunaga Tadashi
Abstract excerpt
An approach to analyze mismatches using short and specific oligonucleotide probes directly synthesized on bacterial magnetic particles (BMPs) by phosphoramidite methods was exploited. Approximately 126 molecules of 4-mer oligonucleotides/particle were synthesized on BMPs with high reaction efficiencies. Hybridization between FITC-labeled oligonucleotides and chemically synthesized oligonucleotides on BMPs was...
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