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Article

Sequence-based typing of HLA-A2 alleles using a primer with an extra base mismatch.

Human immunology - 1 Apr 1995

Ishikawa Y, Tokunaga K, Kashiwase K, Akaza T, Tadokoro K, Juji T

Abstract excerpt

A PCR-SBT method using genomic DNA for HLA-A2 alleles was established. To achieve specific amplification for detecting a single base difference between A2 alleles and other HLA-A alleles, a primer having one extra mismatch at the second position from its 3'-end was designed. The primer exhibited...

Topics

Alleles
Base Composition
Base Sequence
Cytotoxicity Tests, Immunologic
DNA Primers
HLA-A2 Antigen
Histocompatibility Testing
Humans
Molecular Sequence Data
Polymerase Chain Reaction

Source

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Identifiers

PMID: 7558916
DOI: 10.1016/0198-8859(94)00119-b

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-7558916-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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