Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Sequence-based typing of HLA-A2 alleles using a primer with an extra base mismatch.

Human immunology - 1 Apr 1995

Ishikawa Y, Tokunaga K, Kashiwase K, Akaza T, Tadokoro K, Juji T

Abstract excerpt

A PCR-SBT method using genomic DNA for HLA-A2 alleles was established. To achieve specific amplification for detecting a single base difference between A2 alleles and other HLA-A alleles, a primer having one extra mismatch at the second position from its 3'-end was designed. The primer exhibited a high specificity with annealing temperatures from 64 degrees C to 68 degrees C. Thirty-eight Japanese samples were...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Alleles
Share to Base Composition
Share to Base Sequence

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Cytotoxicity Tests, Immunologic

Share to DNA Primers

Share to Histocompatibility Testing

Share to Molecular Sequence Data

Share to Polymerase Chain Reaction

Source

Identifiers

PMID: 7558916
DOI: 10.1016/0198-8859(94)00119-b

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Sequence-based typing of HLA-A2 alleles using a primer with an extra base mismatch.PMID 7558916Read ↗

Sequence of a new HLA-A allele (A*0218) encoding a serological variant, HLA-A2K, observed in Japanese.Semantic proximity Read ↗

DNA typing system for HLA-A2 alleles by polymerase chain reaction with sequence-specific primers.Semantic proximity Read ↗

A PCR-SSP method to specifically select HLA-A*0201 individuals for immunotherapeutic studies.Semantic proximity Read ↗

DNA typing of the HLA-A gene: population study and identification of four new alleles in Japanese.Semantic proximity Read ↗

A nested PCR-RFLP method for high-resolution typing of HLA-A alleles.Semantic proximity Read ↗

HLA-B locus DNA typing: detection of B*7801 and seven additional alleles by BW6-specific exon 2 amplification.Semantic proximity Read ↗

Automated nucleotide sequencing reveals substantial disparity between the HLA-A2 genes of bone marrow transplant recipients and donors.Semantic proximity Read ↗

Allele typing of HLA-A10 group by nested-PCR-low ionic strength single stranded conformation polymorphism and a novel A26 allele (A26KY, A*2605).Semantic proximity Read ↗

DNA typing for HLA class I alleles: I. Subsets of HLA-A2 and of -A28.Semantic proximity Read ↗

Second Allele Finder Software: a Simple Approach Toward HLA Typing.Semantic proximity Read ↗

HLA-A2-binding peptides cross-react not only within the A2 subgroup but also with other HLA-A-locus allelic products.Semantic proximity Read ↗

Sequence analysis of the 2nd intron revealed common sequence motifs providing the means for a unique sequencing based typing protocol of the HLA-A locus.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.