Article
Signal transduction and bacterial conjugation: characterization of the role of ArcA in regulating conjugative transfer of the resistance plasmid R1.
Journal of molecular biology - 27 Mar 1998
Strohmaier H, Noiges R, Kotschan S, Sawers G, Högenauer G, Zechner E L, Koraimann G
Abstract excerpt
The role of the two-component response regulator ArcA protein in the transfer of the conjugative resistance plasmid R1 was investigated using a variety of in vivo and in vitro assays. The frequency of conjugal DNA transfer of plasmid R1-16, a derepressed variant of R1, was reduced by four orders of magnitude in an Escherichia coli host with a mutation in the arcA gene. Measurements of mRNAs transcribed from key...
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