Article
Mutual induced fit binding of Xenopus ribosomal protein L5 to 5S rRNA.
Journal of molecular biology - 25 Jul 2003
DiNitto Jonathan P, Huber Paul W
Abstract excerpt
A library of random mutations in Xenopus ribosomal protein L5 was generated by error-prone PCR and used to delineate the binding domain for 5S rRNA. All but one of the amino acid substitutions that affected binding affinity are clustered in the central region of the protein. Several of the mutations are conservative substitutions of non-polar amino acid residues that are unlikely to form energetically significant...
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