Article
Cooperative assembly of proteins in the ribosomal GTPase centre demonstrated by their interactions with mutant 23S rRNAs.
Nucleic acids research - 11 Jul 1995
Rosendahl G, Douthwaite S
Abstract excerpt
The ribosomal protein L11 binds to the region of 23S rRNA associated with the GTPase-dependent steps of protein synthesis. Nucleotides 1054-1107 within this region of the Escherichia coli 23S rRNA gene were mutagenized with bisulphite. Twenty point mutations (G-->A and C-->T transitions) and numerous multiple mutations were generated. Expression of mutant 23S rRNAs in vivo shows that all the mutations detectably...
Topics
- Base Sequence
- Escherichia coli
- GTP Phosphohydrolases
- Molecular Sequence Data
- Mutagenesis
- Nucleic Acid Conformation
- Phenotype
- Point Mutation
- RNA, Ribosomal, 23S
- Ribosomal Proteins
- Sulfites