Article
Heteromeric MAPPIT: a novel strategy to study modification-dependent protein-protein interactions in mammalian cells.
Nucleic acids research - 15 Jul 2003
Lemmens Irma, Eyckerman Sven, Zabeau Lennart, Catteeuw Dominiek, Vertenten Els, Verschueren Kristin, Huylebroeck Danny, Vandekerckhove Joël, Tavernier Jan
Abstract excerpt
We recently reported a two-hybrid trap for detecting protein-protein interactions in intact mammalian cells (MAPPIT). The bait protein was fused to a STAT recruitment-deficient, homodimeric cytokine receptor and the prey protein to functional STAT recruitment sites. In such a configuration, STAT-dependent responses can be used to monitor a given bait-prey interaction. Using this system, we were able to...
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