Article
A sensitive, quantitative assay for measurement of allele-specific transcripts differing by a single nucleotide.
PCR methods and applications - 1 Feb 1992
Singer-Sam J, LeBon J M, Dai A, Riggs A D
Abstract excerpt
We have found that the single nucleotide primer extension assay, a PCR-based assay currently used qualitatively to measure allelic differences in DNA, can be used quantitatively to measure allele-specific transcripts differing by only a single nucleotide. We show that total RNA containing the Pgk-1a transcript can be specifically detected even in a 1000-fold excess of RNA containing the Pgk-1b transcript.
Topics
- Alleles
- Animals
- Base Sequence
- DNA
- Mice
- Molecular Sequence Data
- Polymerase Chain Reaction
- RNA
- Sensitivity and Specificity
- Transcription, Genetic