Article
A structural, phylogenetic, and functional study of 15.5-kD/Snu13 protein binding on U3 small nucleolar RNA.
RNA (New York, N.Y.) - 1 Jul 2003
Marmier-Gourrier Nathalie, Cléry Antoine, Senty-Ségault Veronique, Charpentier Bruno, Schlotter Florence, Leclerc Fabrice, Fournier Régis, Branlant Christaine
Abstract excerpt
The 15.5-kD protein and its yeast homolog Snu13p bind U4 snRNA, U3 snoRNA, and the C/D box snoRNAs. In U4 snRNA, they associate with a helix-bulge-helix (K-turn) structure. U3 snoRNA contains two conserved pairs of boxes, C'/D and B/C, which were both expected to bind the 15.5-kD/Snu13 protein. Only binding to the B/C motif was experimentally demonstrated. Here, by chemical probing of in vitro reconstituted...
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