Article
Porcine endometrial epithelial cells immortalized by transfection with origin-defective, temperature-sensitive simian virus 40 DNA.
Life sciences - 1 Jan 1992
Li W I, Wu H, Chin M P, Wu G
Abstract excerpt
The purpose of this study was to immortalize porcine endometrial cells and to characterize the transformed cells. Primary porcine endometrial cells were transfected with the plasmid vector (pmk16) containing SV40 DNA using a liposome-mediated method. The viral DNA was from a replication-defective...
Topics
- Animals
- Blotting, Southern
- Cell Division
- Cell Line, Transformed
- Cell Transformation, Viral
- Chromatography, High Pressure Liquid
- DNA, Viral
- Endometrium
- Epithelial Cells
- Epithelium
- Female
- Keratins
- Phenotype
- Simian virus 40
- Swine
- Temperature
- Transfection
- beta-Endorphin