Article
Spreading of Sir3 protein in cells with severe histone H3 hypoacetylation.
Proceedings of the National Academy of Sciences of the United States of America - 24 Jun 2003
Kristjuhan Arnold, Wittschieben Birgitte O, Walker Jane, Roberts Douglas, Cairns Bradley R, Svejstrup Jesper Q
Abstract excerpt
Heterochromatin formation in yeast involves deacetylation of histones, but the precise relationship between acetylation and the association of proteins such as Sir3, Sir4, and the histone deacetylase Sir2 with chromatin is still unclear. Here we show that Sir3 protein spreads to subtelomeric DNA in cells lacking the transcription-related histone acetyltransferases GCN5 and ELP3. Spreading correlates with...
Topics
- Acetylation
- Chromatin
- DNA
- Gene Deletion
- Heterochromatin
- Histone Deacetylases
- Histones
- Lysine
- Oligonucleotide Array Sequence Analysis
- Phenotype
- Precipitin Tests
- Saccharomyces cerevisiae
- Silent Information Regulator Proteins, Saccharomyces cerevisiae