Article
[Peripheral blood mutated p53 DNA and its clinical value in human breast cancer].
Zhonghua zhong liu za zhi [Chinese journal of oncology] - 1 Mar 2003
Di Gen-hong, Liu Gang, Wu Jiong, Shen Zhen-zhou, Shao Zhi-min
Abstract excerpt
OBJECTIVE: To evaluate the clinical value of mutated p53 in the peripheral blood of breast cancer patients. METHODS: Plasma DNA of 126 breast cancer patients and 92 healthy women was examined. DNA extraction from the tumor and tissue samples was performed by a nonorganic method. Plasma DNA was purified on Qiagen columns. PCR-SSCP analysis was performed to examine the point mutations in the conserved exons 5, 6, 7...
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