Article
Analysis of the pyruvate permease gene (JEN1) in glucose derepression yeast (Saccharomyces cerevisiae) Isolated from a 2-deoxyglucose-tolerant mutant, and its application to sake making.
Bioscience, biotechnology, and biochemistry - 1 Apr 2003
Tsuboi Hirokazu, Wakisaka Yasushi, Hirotsune Masato, Akao Takeshi, Yamada Osamu, Akita Osamu
Abstract excerpt
We isolated mutants of S. cerevisiae in which expression of the JEN1 gene encoding a pyruvate transporter was insensitive to glucose repression. The isolated mutant GDR19 expressed JEN1 and absorbed pyruvate in the presence of glucose. In a DNA microarray analysis, GDR19 highly expressed many more genes, including JEN1, in the presence of glucose compared with the parental strain B29. Some of these genes are...
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