Article
Poly(A) tailing of ancient DNA: a method for reproducible microsatellite genotyping.
Analytical biochemistry - 1 Jul 2003
Culjković Biljana, Savić Dusanka, Stojković Oliver, Romac Stanka
Abstract excerpt
Microsatellites could be of great potential use in the analysis of ancient remains, but so far such analyses have failed to be reproducible mainly because of the high degree of ancient DNA (aDNA) degradation. During PCR, annealing of the primers to the complementary sequences of microsatellites occurs together with cross-annealing of partially degraded repeated sequences. This could create chimeric alleles that...
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