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Article

Significantly enhanced stability of glucose dehydrogenase by directed evolution.

Applied microbiology and biotechnology - 1 May 2003

Baik S-H, Ide T, Yoshida H, Kagami O, Harayama S

Abstract excerpt

An NaCl-independent stability-enhanced mutant of glucose dehydrogenase (GlcDH) was obtained by using in vitro directed evolution. The family shuffling method was applied for in vitro directed evolution to construct a mutant library of GlcDH genes. Three GlcDH-coding genes from Bacillus licheniformis IFO 12200, Bacillus megaterium IFO 15308 and Bacillus subtilis IFO 13719 were each cloned by direct PCR...

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Identifiers

PMID: 12743762
DOI: 10.1007/s00253-002-1215-1

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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