Article
Comparison of competitively primed and conventional allele-specific nucleic acid amplification.
Analytical biochemistry - 15 May 2001
Giffard P M, McMahon J A, Gustafson H M, Barnard R T, Voisey J
Abstract excerpt
A simulation of competitively primed allele-specific DNA amplification has been constructed and its behavior examined. This has shown that when the ratio of the amount of homoduplex misprime product to the total amount of amplimer is low, it increases by approximately one-fourth of the mispriming frequency with each doubling of the total amount of amplimer. When the ratio is high and reverse mispriming becomes...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.