Technical aspects of typing for HLA-DP alleles using allele-specific DNA in vitro amplification and sequence-specific oligonucleotide probes. Detection of single base mismatches.

Abstract excerpt

The polymerase chain reaction (PCR) is an effective method for in vitro DNA amplification which combined with probing with synthetic oligonucleotides can be used for, e.g., HLA-typing. We have studied the technical aspects of HLA-DP typing with the technique. DNA from mononuclear nucleated cells was extracted with either a simple salting out method or phenol/chloroform. Both DNAs could be readily used for PCR....

Topics

• Alleles
• Amino Acid Sequence
• Base Sequence
• DNA Probes, HLA
• Genetic Techniques
• Genetic Variation
• HLA-DP Antigens
• Humans
• Magnesium Chloride
• Molecular Sequence Data