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Article

Purification and characterisation of a lactococcal aminoacylase.

Archives of microbiology - 1 Jun 2003

Curley Peter, van der Does Chris, Driessen Arnold J M, Kok Jan, van Sinderen D

Abstract excerpt

The amd1-encoded aminoacylase from Lactococcus lactis MG1363 was cloned and overexpressed in Escherichia coli and purified. The assumed dimeric enzyme has a subunit molecular mass of about 42 kDa and contains 2.0+/-0.1 g-atoms of zinc and cobalt, in equimolar amounts, per subunit of Amd1. The enzyme was characterised with respect to substrate specificity, pH, temperature and metal dependence. Amd1 exhibited a...

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Identifiers

PMID: 12682714
DOI: 10.1007/s00203-003-0544-5

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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