Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

TnAraOut, a transposon-based approach to identify and characterize essential bacterial genes.

Nature biotechnology - 1 Jul 2000

Judson N, Mekalanos J J

Abstract excerpt

Identification of genes that encode essential products provides a promising approach to validation of new antibacterial drug targets. We have developed a mariner-based transposon, TnAraOut, that allows efficient identification and characterization of essential genes by transcriptionally fusing them to an outward-facing, arabinose-inducible promoter, PBAD, located at one end of the transposon. In the absence of...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Arabinose
Share to beta-Galactosidase
Share to Cell Culture Techniques

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Cell Division

Share to DNA Transposable Elements

Share to Dose-Response Relationship, Drug

Share to Mutagenesis

Share to Vibrio cholerae

Source

Identifiers

PMID: 10888841
DOI: 10.1038/77305

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

TnAraOut, a transposon-based approach to identify and characterize essential bacterial genes.PMID 10888841Read ↗

Essential genes in Salmonella enteritidis as identified by TnAraOut mutagenesis.Semantic proximity Read ↗

From microbial gene essentiality to novel antimicrobial drug targetsSemantic proximity Read ↗

High-throughput transposon mutagenesis in the family Enterobacteriaceae reveals core essential genes and rapid turnover of essentialitySemantic proximity Read ↗

A genome-scale analysis for identification of genes required for growth or survival of Haemophilus influenzae.Semantic proximity Read ↗

Application of TraDIS to define the core essential genome of Campylobacter jejuni and Campylobacter coliSemantic proximity Read ↗

Identification of Essential Protein Domains From High-density Transposon Insertion SequencingSemantic proximity Read ↗

Genome-Wide Essentiality Analysis of Mycobacterium abscessus by Saturated Transposon Mutagenesis and Deep SequencingSemantic proximity Read ↗

Comparison of inherently essential genes of Porphyromonas gingivalis identified in two transposon-sequencing libraries.Semantic proximity Read ↗

Scanning the Escherichia coli chromosome by random transposon mutagenesis and multiple phenotypic screening.Semantic proximity Read ↗

The Essential Genome of Burkholderia cenocepacia H111.Semantic proximity Read ↗

Importance of Core Genome Functions for an Extreme Antibiotic Resistance Trait.Semantic proximity Read ↗

Candidate Essential Genes in Burkholderia cenocepacia J2315 Identified by Genome-Wide TraDISSemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.