Article
Identification of crucial residues of conformational epitopes on VP2 protein of infectious bursal disease virus by phage display.
Journal of virological methods - 1 Apr 2003
Cui Xianlan, Nagesha Hadya S, Holmes Ian H
Abstract excerpt
A new method for identifying epitopes in viral proteins expressed by filamentous phage has been developed. Filamentous phage fUSE 1 containing the variable region of the VP2 gene of infectious bursal disease virus (IBDV) strain 002-73 was constructed. Neutralizing monoclonal antibodies 17-82 and 33-10 raised against VP2 protein were used to bind phage containing the original variable region of VP2. The phage...
Topics
- Animals
- Antibodies, Monoclonal
- Antibodies, Viral
- Chickens
- Coliphages
- Epitope Mapping
- Epitopes
- Escherichia coli
- Gene Library
- Infectious bursal disease virus
- Mutation
- Neutralization Tests
- Protein Conformation
- Viral Structural Proteins
