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Article

The effect of pressure and guanidine hydrochloride on azurins mutated in the hydrophobic core.

European journal of biochemistry - 1 Oct 1999

Mei G, Di Venere A, Campeggi F M, Gilardi G, Rosato N, De Matteis F, Finazzi-Agrò A

Abstract excerpt

The unfolding of the blue-copper protein azurin from Pseudomonas aeruginosa by guanidine hydrochloride, under nonreducing conditions, has been studied by fluorescence techniques and circular dichroism. The denaturation transition may be fitted by a simple two-state model. The total free energy change from the native to the unfolded state was 9.4 +/- 0.4 kcal.mol-1, while a lower value (6.4 +/- 0.4 kcal.mol-1) was...

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Source

Identifiers

PMID: 10504393
DOI: 10.1046/j.1432-1327.1999.00751.x

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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