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Article

Targeted nucleotide repair of cyc1 mutations in Saccharomyces cerevisiae directed by modified single-stranded DNA oligonucleotides.

Genetics - 1 Feb 2003

Brachman Erin E, Kmiec Eric B

Abstract excerpt

Modified single-stranded DNA oligonucleotides have been used to direct base changes in the CYC1 gene of Saccharomyces cerevisiae. In this process, the oligonucleotide is believed to hybridize to the target site through the action of a DNA recombinase and, once bound, DNA repair enzymes act to excise the nucleotide, replace it, and revert the gene to wild-type status. Nucleotide exchange exhibits a strand bias as,...

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Identifiers

PMID: 12618392
DOI: 10.1093/genetics/163.2.527
PMCID: PMC1462467

Genes

CYC1

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Source receipt: pubmed, retrieved 7 Sept 2026.

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