Article
Strand-specificity in the transformation of yeast with synthetic oligonucleotides.
Genetics - 1 Aug 1992
Yamamoto T, Moerschell R P, Wakem L P, Komar-Panicucci S, Sherman F
Abstract excerpt
Cyc1 mutants of the yeast Saccharomyces cerevisiae were directly transformed with both sense and antisense oligonucleotides to examine the involvement of the two genomic DNA strands in transformation. Sense oligonucleotides yielded approximately 20-fold more transformants than antisense oligonucl...
Topics
- Alleles
- Amino Acid Sequence
- Base Sequence
- Cytochrome c Group
- Cytochromes c
- DNA, Antisense
- DNA, Fungal
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Oligodeoxyribonucleotides
- Saccharomyces cerevisiae
- Saccharomyces cerevisiae Proteins