Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Metabolic flux profiling of Escherichia coli mutants in central carbon metabolism using GC-MS.

European journal of biochemistry - 1 Mar 2003

Fischer Eliane, Sauer Uwe

Abstract excerpt

We describe here a novel methodology for rapid diagnosis of metabolic changes, which is based on probabilistic equations that relate GC-MS-derived mass distributions in proteinogenic amino acids to in vivo enzyme activities. This metabolic flux ratio analysis by GC-MS provides a comprehensive perspective on central metabolism by quantifying 14 ratios of fluxes through converging pathways and reactions from...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Carbon
Share to Carbon Isotopes
Share to Escherichia coli

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Gas Chromatography-Mass Spectrometry

Share to Mutation

Source

Identifiers

PMID: 12603321
DOI: 10.1046/j.1432-1033.2003.03448.x

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Metabolic flux profiling of Escherichia coli mutants in central carbon metabolism using GC-MS.PMID 12603321Read ↗

Systematic phenome analysis of Escherichia coli multiple-knockout mutants reveals hidden reactions in central carbon metabolism.Semantic proximity Read ↗

A kinetic model of Escherichia coli core metabolism satisfying multiple sets of mutant flux data.Semantic proximity Read ↗

<sup>13</sup> C flux ratio analysis with FRAPPPE reveals differences in metabolic fluxes between gut Bacteroidota and <i>Escherichia coli</i>Semantic proximity Read ↗

Metabolite essentiality elucidates robustness of Escherichia coli metabolism.Semantic proximity Read ↗

Flux-sum analysis: a metabolite-centric approach for understanding the metabolic network.Semantic proximity Read ↗

Analysis of Escherichia coli anaplerotic metabolism and its regulation mechanisms from the metabolic responses to altered dilution rates and phosphoenolpyruvate carboxykinase knockout.Semantic proximity Read ↗

13C-metabolic flux analysis for batch culture of Escherichia coli and its Pyk and Pgi gene knockout mutants based on mass isotopomer distribution of intracellular metabolites.Semantic proximity Read ↗

Construction and evolution of an Escherichia coli strain relying on nonoxidative glycolysis for sugar catabolism.Semantic proximity Read ↗

Metabolic flux analysis for a ppc mutant Escherichia coli based on 13C-labelling experiments together with enzyme activity assays and intracellular metabolite measurements.Semantic proximity Read ↗

Omic data from evolved E. coli are consistent with computed optimal growth from genome-scale models.Semantic proximity Read ↗

Metabolic flux balance analysis and the in silico analysis of Escherichia coli K-12 gene deletions.Semantic proximity Read ↗

Identification of genome-scale metabolic network models using experimentally measured flux profiles.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.