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Article

Mutant luciferase enzymes from fireflies with increased resistance to benzalkonium chloride.

Bioscience, biotechnology, and biochemistry - 1 Dec 2002

Hattori Noriaki, Kajiyama Naoki, Maeda Masako, Murakami Seiji

Abstract excerpt

Benzalkonium chloride (BAC), used to extract intracellular ATP, interferes with subsequent firefly luciferase-luciferin assays. There was a significant difference among wild-type luciferases with respect to BAC resistance. Luciola lateralis luciferase (LlL) was the most tolerant, followed by Luciola cruciata luciferase (LcL) and Photinus pyralis luciferase. Random mutagenesis of thermostable mutants of LcL showed...

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Identifiers

PMID: 12596852
DOI: 10.1271/bbb.66.2587

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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