Article
The Hansenula polymorpha PER1 gene is essential for peroxisome biogenesis and encodes a peroxisomal matrix protein with both carboxy- and amino-terminal targeting signals.
The Journal of cell biology - 1 Nov 1994
Waterham H R, Titorenko V I, Haima P, Cregg J M, Harder W, Veenhuis M
Abstract excerpt
We describe the cloning of the Hansenula polymorpha PER1 gene and the characterization of the gene and its product, PER1p. The gene was cloned by functional complementation of a per1 mutant of H. polymorpha, which was impaired in the import of peroxisomal matrix proteins (Pim- phenotype). The DNA...
Topics
- Amino Acid Sequence
- Base Sequence
- Blotting, Northern
- Cloning, Molecular
- DNA Primers
- DNA, Fungal
- Fungal Proteins
- Genes, Fungal
- Genetic Complementation Test
- Microbodies
- Microscopy, Electron