Article
Isolation of mutants of Hansenula polymorpha defective in the assembly of octameric alcohol oxidase.
FEMS yeast research - 1 Jan 2002
van Dijk Ralf, Lahchev Kancho L, Kram Anita M, van der Klei Ida J, Veenhuis Marten
Abstract excerpt
Alcohol oxidase (AO) is a peroxisomal enzyme that catalyses the first step in methanol metabolism in yeast. Monomeric, inactive AO protein is synthesised in the cytosol and subsequently imported into peroxisomes, where the enzymatically active, homo-octameric form is found. The mechanisms involved in AO octamer assembly are largely unclear. Here we describe the isolation of Hansenula polymorpha mutants...
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