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Trapping HIV-1 Reverse Transcriptase Before and After Translocation on DNA

25 Apr 2003

Abstract excerpt

A disulfide cross-linking strategy was used to covalently trap as a stable complex (complex N) a short-lived, kinetic intermediate in DNA polymerization. This intermediate corresponds to the product of polymerization prior to translocation. We also prepared the trapped complex that corresponds to the product of polymerization after translocation (complex P). The cross-linking method that we used is a variation of...

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PMID: 12554739
DOI: 10.1074/jbc.m212911200

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