Article
Flow cytometric quantification of apoptosis and proliferation in mixed lymphocyte culture.
Cytometry. Part A : the journal of the International Society for Analytical Cytology - 1 Feb 2003
Marín Luis, Minguela Alfredo, Torío Alberto, Moya-Quiles María R, Muro Manuel, Montes-Ares Olga, Parrado Antonio, Alvarez-López D María Rocío, García-Alonso Ana M
Abstract excerpt
BACKGROUND: The one-way mixed lymphocyte culture (MLC) is the classic culture used for studying the allogenic immunoresponse in vitro, but stimulator and responder cell identifications and quantification of apoptotic or proliferative responder cells are unreliable. METHODS: Peripheral blood mononuclear cells were labeled with 5- (and 6-) carboxy fluorescein diacetate succinimidyl ester (CFSE) and stimulated with...
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