Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

A new four-color flow cytometric assay to detect apoptosis in lymphocyte subsets of cultured peripheral blood cells

1 Jun 2000

Abstract excerpt

BACKGROUND: Human peripheral blood lymphocytes kept in culture after isolation die by an apoptotic process. Detection of apoptosis with labeled Annexin V to demonstrate loss of plasma membrane asymmetry is sensitive, specific, and easy using flow cytometry. This is true in lymphoblastic cell lines when combining Annexin V-fluorescein isothiocyanate (FITC) and propidium iodide (PI). However, measurement of...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Cell death mechanisms and regulation
Share to Erythrocyte Function and Pathophysiology
Share to Phagocytosis and Immune Regulation

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Source

Identifiers

PMID: 10805937
DOI: 10.1002/(sici)1097-0320(20000601)40:2<167::aid-cyto11>3.0.co;2-1

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

A new four-color flow cytometric assay to detect apoptosis in lymphocyte subsets of cultured peripheral blood cellsPMID 10805937Read ↗

Novel approach for simultaneous evaluation of cell phenotype, apoptosis, and cell cycle using multiparameter flow cytometry.Semantic proximity Read ↗

Colourful death: Six-parameter classification of cell death by flow cytometry—Dead cells tell talesSemantic proximity Read ↗

A six-colour flow cytometric method for simultaneous detection of cell phenotype and apoptosis of circulating endothelial cells.Semantic proximity Read ↗

A rapid ex vivo clinical diagnostic assay for fas receptor-induced T lymphocyte apoptosis.Semantic proximity Read ↗

Resistance to apoptosis correlates with a highly proliferative phenotype and loss of Fas and CPP32 (caspase-3) expression in human leukemia cells.Semantic proximity Read ↗

Comparative measurement of spontaneous apoptosis in pediatric acute leukemia by different techniquesSemantic proximity Read ↗

Distinct mechanisms trigger apoptosis in human immunodeficiency virus type 1-infected and in uninfected bystander T lymphocytes.Semantic proximity Read ↗

Methods for simultaneous measurement of apoptosis and cell surface phenotype of epithelial cells in effusions by flow cytometry.Semantic proximity Read ↗

Spontaneous apoptosis in human thymocytes.Semantic proximity Read ↗

Spontaneous apoptosis of blood dendritic cells in patients with breast cancer.Semantic proximity Read ↗

Flow cytometric analysis of live cell proliferation and phenotype in populations with low viability.Semantic proximity Read ↗

Effects of annexin A1 on apoptosis and cell cycle arrest in human leukemic cell linesSemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.