Article
Flow cytometric analysis of live cell proliferation and phenotype in populations with low viability.
Cytometry - 1 Jan 1999
Schmid I, Ferbas J, Uittenbogaart C H, Giorgi J V
Abstract excerpt
BACKGROUND: Combined analysis of DNA content and immunofluorescence on single cells by flow cytometry provides information on the proliferative response of subpopulations to stimuli in mixed cell preparations; however, in low-viability cell preparations, dead cells interfere with accurate flow cytometric data analysis because of nonspecific binding of antibodies and altered DNA-staining profiles. Light scatter...
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