Flow cytometric analysis of live cell proliferation and phenotype in populations with low viability.

doi:10.1002/(sici)1097-0320(19990101)35:1<64::aid-cyto9>3.3.co;2-p

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Article

Flow cytometric analysis of live cell proliferation and phenotype in populations with low viability.

Cytometry - 1 Jan 1999

Schmid I, Ferbas J, Uittenbogaart C H, Giorgi J V

Abstract excerpt

BACKGROUND: Combined analysis of DNA content and immunofluorescence on single cells by flow cytometry provides information on the proliferative response of subpopulations to stimuli in mixed cell preparations; however, in low-viability cell preparations, dead cells interfere with accurate flow cytometric data analysis because of nonspecific binding of antibodies and altered DNA-staining profiles. Light scatter...

Topics

Cell Death
Cell Division
Cell Survival
Coloring Agents
DNA, Neoplasm
Dactinomycin
Flow Cytometry
Fluorescent Antibody Technique
Fluorescent Dyes
Humans
Leukemia, T-Cell
Leukocytes, Mononuclear
Phenotype
Propidium
Pyronine
Tumor Cells, Cultured

Source

Open in PubMed

Identifiers

PMID: 10554182
DOI: 10.1002/(sici)1097-0320(19990101)35:1<64::aid-cyto9>3.3.co;2-p

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