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Article

Polymerase chain reaction fidelity and denaturing high-performance liquid chromatography.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences - 25 Dec 2002

Muhr Daniela, Wagner Teresa, Oefner Peter J

Abstract excerpt

Incorporation of non-complementary nucleotides during polymerase chain reaction can result in ambiguous denaturing high-performance liquid chromatography profiles that reduce both sensitivity and specificity of mutation analysis. The use of proofreading DNA polymerases increases the fidelity of polymerase chain reaction and, consequently, reduces background noise in the chromatograms. This is demonstrated for...

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Source

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PMID: 12458000
DOI: 10.1016/s1570-0232(02)00695-5

Genes

BRCA1
BRCA2

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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