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Article

Role of an intrasubunit disulfide in the association state of the cytosolic homo-oligomer methionine adenosyltransferase.

The Journal of biological chemistry - 28 Feb 2003

Sanchez-Perez Gabino F, Gasset Maria, Calvete Juan J, Pajares Maria A

Abstract excerpt

Recombinant rat liver methionine adenosyltransferase has been refolded into fully active tetramers (MAT I) and dimers (MAT III), using as a source chaotrope-solubilized aggregates resulting from specific washes of inclusion bodies. The conditions of refolding, dialysis in the presence of 10 mm dithiothreitol or 10 mm GSH with 1 mm GSSG, allowed the production of both isoforms, the nature of the redox agent...

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PMID: 12496263
DOI: 10.1074/jbc.M210177200

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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