Article
Active site serine promotes stabilization of the reactive glutathione thiolate in rat glutathione transferase T2-2. Evidence against proposed sulfatase activity of the corresponding human enzyme.
The Journal of biological chemistry - 24 Mar 2000
Jemth P, Mannervik B
Abstract excerpt
Ser(11) in rat glutathione transferase T2-2 is important for stabilization of the reactive enzyme-bound glutathione thiolate in the reaction with 1-menaphthyl sulfate. The S11A mutation increased the pK(a) value for the pH dependence of the rate constant for pre-steady-state product formation, fr...
Topics
- Animals
- Catalytic Domain
- Glutathione
- Glutathione Transferase
- Humans
- Hydrogen-Ion Concentration
- Infant, Newborn
- Kinetics
- Mercaptoethanol
- Models, Chemical
- Mutation
- Nitrobenzenes
- Rats
- Serine
- Sulfatases
- Viscosity