Article
Single nucleotide polymorphism genotyping by on-line liquid chromatography-mass spectrometry in forensic science of the Y-chromosomal locus M9.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences - 25 Dec 2002
Berger Burkhard, Hölzl Georg, Oberacher Herbert, Niederstätter Harald, Huber Christian G, Parson Walther
Abstract excerpt
A method is described for genotyping alleles of the Y-chromosomal locus M9, incorporating DNA extraction, amplification by polymerase chain reaction (PCR), sample purification by ion-pair reversed-phase high-performance liquid chromatography (IP-RP-HPLC), and allele identification by on-line hyphenation to electrospray ionization mass spectrometry (ESI-MS). The alleles G and C were differentiated in 114 base pair...
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