Article
Genotyping by mass spectrometric analysis of short DNA fragments.
Nature biotechnology - 1 Dec 1998
Laken S J, Jackson P E, Kinzler K W, Vogelstein B, Strickland P T, Groopman J D, Friesen M D
Abstract excerpt
A method has been developed to produce small DNA fragments from PCR products for analysis of defined DNA variations by mass spectrometry. The genomic region to be analyzed is PCR-amplified with primers containing a sequence for the type IIS restriction endonuclease Bpml. Bpml digestion of the res...
Topics
- Base Sequence
- Codon
- DNA
- Genes, APC
- Genotype
- Humans
- Mass Spectrometry
- Polymerase Chain Reaction
- Restriction Mapping