Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Optimized suppression of adducts in polymerase chain reaction products for semi-quantitative SNP genotyping by liquid chromatography-mass spectrometry.

Journal of the American Society for Mass Spectrometry - 1 Dec 2004

Oberacher Herbert, Parson Walther, Hölzl Georg, Oefner Peter J, Huber Christian G

Abstract excerpt

While electrospray ionization mass spectrometry has shown great potential for the identification of genotypes in DNA sequences amplified by polymerase chain reaction (PCR), the quantitative determination of allele frequencies remains challenging because of the presence of cationic adducts in the mass spectra which severely impairs accuracy of quantitation. We report here on the elaboration of an optimized...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Chromatography, High Pressure Liquid
Share to Chromosomes, Human, Y

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to DNA Adducts

Share to Genotype

Share to Polymerase Chain Reaction

Share to Polymorphism, Single Nucleotide

Share to Spectrometry, Mass, Electrospray Ionization

Source

Identifiers

PMID: 15589766
DOI: 10.1016/j.jasms.2004.09.004

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Optimized suppression of adducts in polymerase chain reaction products for semi-quantitative SNP genotyping by liquid chromatography-mass spectrometry.PMID 15589766Read ↗

Re-sequencing of multiple single nucleotide polymorphisms by liquid chromatography-electrospray ionization mass spectrometry.Semantic proximity Read ↗

A novel amplification strategy for genotyping with liquid chromatography-electrospray ionization mass spectrometry.Semantic proximity Read ↗

Analysis of single nucleotide polymorphisms by primer extension and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.Semantic proximity Read ↗

Analysis of polymerase chain reaction products by on-line liquid chromatography-mass spectrometry for genotyping of polymorphic short tandem repeat loci.Semantic proximity Read ↗

SNP genotyping using alkali cleavage of RNA/DNA chimeras and MALDI time-of-flight mass spectrometry.Semantic proximity Read ↗

Detection of DNA sequence variations in homo- and heterozygous samples via molecular mass measurements by electrospray ionization time-of-flight mass spectrometry.Semantic proximity Read ↗

Evaluation of the LTQ-Orbitrap mass spectrometer for the analysis of polymerase chain reaction products.Semantic proximity Read ↗

Mutation scanning by ion-pair reversed-phase high-performance liquid chromatography-electrospray ionization mass spectrometry (ICEMS).Semantic proximity Read ↗

Profiling 627 mitochondrial nucleotides via the analysis of a 23-plex polymerase chain reaction by liquid chromatography-electrospray ionization time-of-flight mass spectrometry.Semantic proximity Read ↗

Genotyping single nucleotide polymorphisms using intact polymerase chain reaction products by electrospray quadrupole mass spectrometry.Semantic proximity Read ↗

Automated solid-phase extraction for purification of single nucleotide polymorphism genotyping products prior to matrix-assisted laser desorption/ionisation time-of-flight mass spectrometric analysis.Semantic proximity Read ↗

The next generation of DNA profiling--STR typing by multiplexed PCR--ion-pair RP LC-ESI time-of-flight MS.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.