Article
Direct detection and automated sequencing of individual alleles after electrophoretic strand separation: identification of a common nonsense mutation in exon 9 of the human lipoprotein lipase gene.
Nucleic acids research - 25 Sept 1990
Hata A, Robertson M, Emi M, Lalouel J M
Abstract excerpt
Large-scale screening by direct sequencing of DNA to detect molecular variants remains a laborious endeavor whose difficulty is compounded by heterozygosity. We show that mobility shifts of single-stranded DNA electrophoresed under nondenaturing conditions can be used not only to detect variants (Orita,M. et al., 1989, Genomics, 5, 874-879), but also to separate and sequence directly individual alleles. In this...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.