Direct detection and automated sequencing of individual alleles after electrophoretic strand separation: identification of a common nonsense mutation in exon 9 of the human lipoprotein lipase gene.

doi:10.1093/nar/18.18.5407

Article

Direct detection and automated sequencing of individual alleles after electrophoretic strand separation: identification of a common nonsense mutation in exon 9 of the human lipoprotein lipase gene.

Nucleic acids research - 25 Sept 1990

Hata A, Robertson M, Emi M, Lalouel J M

Abstract excerpt

Large-scale screening by direct sequencing of DNA to detect molecular variants remains a laborious endeavor whose difficulty is compounded by heterozygosity. We show that mobility shifts of single-stranded DNA electrophoresed under nondenaturing conditions can be used not only to detect variants...

Topics

Alleles
Amino Acid Sequence
Base Sequence
Electrophoresis
Exons
Genes
Heterozygote
Humans
Hypertriglyceridemia
Lipoprotein Lipase
Molecular Sequence Data
Mutation
Polymerase Chain Reaction

Direct detection and automated sequencing of individual alleles after electrophoretic strand separation: identification of a common nonsense mutation in exon 9 of the human lipoprotein lipase gene.

Abstract excerpt

Topics

Discuss the evidence