Article
[Mutations in beta'-subunit of the Escherichia coli RNA-polymerase influence interaction with downstream duplex DNA in the elongation complex].
Genetika - 1 Oct 2002
Kul'bachinskiĭ A V, Ershova G V, Korzheva N V, Brodolin K L, Nikiforov V G
Abstract excerpt
RNA polymerase (RNAP) exhibits absolute processivity being capable of synthesizing RNA 10(3)-10(5) nucleotides in length without breaking contact with the DNA template. Stability of the elongation complex is thought to depend, in particular, on the RNAP-DNA interactions downstream along the run of transcription. We studied the effects of several deletions and insertions in the RNAP beta'-subunit N-terminal...
Topics
- Amino Acid Sequence
- DNA-Directed RNA Polymerases
- Escherichia coli Proteins
- Molecular Sequence Data
- Mutation
- Nucleic Acid Heteroduplexes
- Peptide Chain Elongation, Translational
- Promoter Regions, Genetic
- Sequence Homology, Amino Acid