Article
Genotyping of hepatitis C virus by melting curve analysis with SYBR Green I.
Annals of clinical biochemistry - 1 Mar 2004
Fujigaki Hidetsugu, Takemura Masao, Takahashi Kanako, Yamada Yasuhiro, Fujii Hidehiko, Wada Hisayasu, Saito Kuniaki, Ohnishi Hiroo, Seishima Mitsuru
Abstract excerpt
BACKGROUND: Recent studies have focused on whether different hepatitis C virus (HCV) genotypes are associated with different profiles of pathogenicity, infectivity, and response to antiviral therapy. We needed to develop a convenient screening test for HCV genotypes 1 and 2. METHOD: We tested 55 patients with known chronic HCV infection. Viral RNA was extracted from serum samples using an automatic viral RNA...
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