Article
High-throughput measurement of mitochondrial membrane potential in a neural cell line using a fluorescence plate reader.
Biochemical and biophysical research communications - 15 Nov 2002
Wong Alice, Cortopassi Gino A
Abstract excerpt
Mutations in mitochondrial genes cause mitochondrial genetic disease, which is often associated with deficiency of the mitochondrial membrane potential (MMP). We present a high-throughput method for measuring MMP in intact neural cells using TMRM, a well-known potentiometric dye, in a 48-well plate format. Addition of known MMP depolarizing agents, FCCP or DNP, resulted in a time- and concentration-dependent...
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