Article
Construction of green fluorescent protein (GFP)-marked strains of Bradyrhizobium for ecological studies.
Journal of applied microbiology - 1 Jan 2002
Bhatia R, Dogra R C, Sharma P K
Abstract excerpt
AIM: To introduce the gfp gene encoding green fluorescent protein (GFP) into bradyrhizobia for their identification in nodules, soil and carrier-based inoculants. METHODS AND RESULTS: Bradyrhizobium sp. strains M29 and GN7, which nodulate mungbean (Vigna radiata), were conjugated with Escherichia coli S17-1 carrying plasmid EDS 15 (a suicide plasmid carrying a promoterless gfp gene fused with Tn5). The GFP-marked...
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