Article
Easy detection of green fluorescent protein multicopy transformants in Penicillium griseoroseum.
Genetics and molecular research : GMR - 30 Dec 2004
Lopes Francis J F, de Araújo Elza F, de Queiroz Marisa V
Abstract excerpt
Penicillium griseoroseum, a deuteromycete fungus producer of pectinolytic enzymes, was transformed with a gene encoding for green fluorescent protein (GFP). The selection of transformants was based on the homologous nitrate reductase gene (niaD). Protoplasts of a P. griseoroseum Nia mutant (PG63) were co-transformed with the plasmids pNPG1 and pAN52-1-GFP. The plasmid pNPG-1 carries the homologous niaD gene and...
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