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A pyrF auxotrophic mutant of Sinorhizobium fredii HH103 impaired in its symbiotic interactions with soybean and other legumes.

International microbiology : the official journal of the Spanish Society for Microbiology - 1 Sept 2007

Crespo-Rivas Juan C, Margaret Isabel, Pérez-Montaño Francisco, López-Baena Francisco J, Vinardell José M, Ollero Francisco J, Moreno Javier, Ruiz-Sainz José E, Buendía-Clavería Ana M

Abstract excerpt

Transposon Tn5-Mob mutagenesis allowed the selection of a Sinorhizobium fredii HH103 mutant derivative (SVQ 292) that requires the presence of uracil to grow in minimal media. The mutated gene, pyrF, codes for an orotidine-5 - monophosphate decarboxylase (EC 4.1.1.23). Mutant SVQ 292 and its parental prototrophic mutant HH103 showed similar Nod-factor and lipopolysaccharide profiles. The symbiotic properties of...

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PMID: 18075998

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A pyrF auxotrophic mutant of Sinorhizobium fredii HH103 impaired in its symbiotic interactions with soybean and other legumes.PMID 18075998Read ↗

Construction and pilot screening of a signature-tagged mutant library of Sinorhizobium fredii.Semantic proximity Read ↗

A purL mutant of Sinorhizobium fredii HH103 is symbiotically defective and altered in its lipopolysaccharide.Semantic proximity Read ↗

Sinorhizobium fredii HH103 does not strictly require KPS and/or EPS to nodulate Glycyrrhiza uralensis, an indeterminate nodule-forming legume.Semantic proximity Read ↗

Mutation in the cobO gene generates auxotrophy for cobalamin and methionine and impairs the symbiotic properties of Sinorhizobium fredii HH103 with soybean and other legumes.Semantic proximity Read ↗

Sinorhizobium fredii HH103 cgs mutants are unable to nodulate determinate- and indeterminate nodule-forming legumes and overproduce an altered EPS.Semantic proximity Read ↗

Mutation in GDP-fucose synthesis genes of Sinorhizobium fredii alters Nod factors and significantly decreases competitiveness to nodulate soybeans.Semantic proximity Read ↗

The effect of FITA mutations on the symbiotic properties of Sinorhizobium fredii varies in a chromosomal-background-dependent manner.Semantic proximity Read ↗

A nopA deletion mutant of Sinorhizobium fredii USDA257, a soybean symbiont, is impaired in nodulation.Semantic proximity Read ↗

Sinorhizobium fredii HH103 mutants affected in capsular polysaccharide (KPS) are impaired for nodulation with soybean and Cajanus cajan.Semantic proximity Read ↗

Structure and biological roles of Sinorhizobium fredii HH103 exopolysaccharide.Semantic proximity Read ↗

The Sinorhizobium fredii HH103 lipopolysaccharide is not only relevant at early soybean nodulation stages but also for symbiosome stability in mature nodules.Semantic proximity Read ↗

Sinorhizobium fredii HH103 syrM inactivation affects the expression of a large number of genes, impairs nodulation with soybean and extends the host-range to Lotus japonicus.Semantic proximity Read ↗

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